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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: DNA Repair (Amst). 2011 Oct 29;10(12):1282–1293. doi: 10.1016/j.dnarep.2011.10.008

Figure 4. XRCC1 facilitates AAG-mediated NO· -induced base lesion removal.

Figure 4

(A) Immunoblot showing expression of AAG (MPG) and XRCC1 protein in wild type (LN428WT), AAG over-expressing cells (AAGOE), XRCC1 knockdown cells (XRCC1-KD), and AAG over-expressing cells with XRCC1 knocked down (AAGOE/XRCC1-KD). (B) Bar graph showing the relative protein expression of AAG and XRCC1 in LN428WT, AAGOE, XRCC1-KD and AAGOE/XRCC1-KD cells. The immunoblot in (A) was scanned and quantified using NIH Image J and the associated analysis software package, normalizing the expression across the four cell lines to the AAGOE cells and to the expression of PCNA within each sample. (C–F) Fluorescent molecular beacon assay indicating XRCC1 facilitated excision of NO· -induced base lesions by AAG from DNA double-stranded oligonucleotides containing (C) no lesion, (D) an abasic analog THF, (E) hypoxanthine (Hx) or (F) 1,N6-ethenoadenine (εA).