Table 1.
Critical micelle concentration (CMC), hydrodynamic radii (Rh) of the micelles (mean ± SD, n = 5) and protein solubilization yields for CGT amphiphiles (CGT-2 and CGT-3), previously described TPAs (TPA-2 and TPA-4) and three conventional detergents (DDM, TX-100 and LDAO).
| MWa | CMC (mM) |
CMC (wt %) |
Rh (nm)b | SY (%)c | |
|---|---|---|---|---|---|
| CGT-2 | 661.7 | ~2.6 | ~0.17 | 2.6 ± 0.10 | ~30 |
| CGT-3 | 631.7 | ~1.9 | ~0.12 | 5.4 ± 0.09 | ~85 |
| TPA-2 | 659.8 | ~3.6 | ~0.24 | 2.0 ± 0.06 | ~50 |
| TPA-4 | 629.7 | ~4.0 | ~0.25 | N.D. | ~95 |
| DDM | 510.1 | ~0.17 | ~0.0087 | 3.5 ± 0.04 | ~70 |
| TX-100 | ~625 | ~0.22 | ~0.01 | 4.2d | > 95 |
| LDAO | 229.4 | 1-2 | ~0.023 | 2.0d | ~100 |
a
Molecular weight of detergents.
b
Hydrodynamic radius of micelles as determined at 0.5 wt %. TPA-2 was used at 1.0 wt % to obtain strong signal.
c
Solubilization yield of R. capsulatus photosynthetic superassembly from the native intracytoplasmic membranes.
d
These values were obtained from the literatures.14
N.D. = Not Determined.