Table 1.
Peptide Sequence (heavy-labeled) | Protein Name | Species | Modified residue(s) | Precursor ion m/z | z | Product ion m/z | Fragment identity | a Frag (V) | b CE (V) | c CV (%) |
---|---|---|---|---|---|---|---|---|---|---|
AApYFGVYDTAK | ANT1 (ADP/ATP translocase 1) | d M, e H | Y191 | 647.3 | 2+ | 908.5 | y8 | 180 | 16 | 7.1 |
M, H | 647.3 | 2+ | 761.4 | y7 | 180 | 20 | 8.6 | |||
M, H | 647.3 | 2+ | 386.1 | b3 | 180 | 20 | 6.9 | |||
| ||||||||||
DFLAGGIAAAVpSK | ANT1 (ADP/ATP translocase 1) | M | S22 | 654.3 | 2+ | 861.4 | y9 | 155 | 12 | 3.2 |
M | 654.3 | 2+ | 834.5 | y10-H3PO4 | 155 | 24 | 0.7 | |||
M | 654.3 | 2+ | 263.1 | b2 | 155 | 20 | 7.7 | |||
M | 654.3 | 2+ | 235.1 | a2 | 155 | 36 | 7.9 | |||
| ||||||||||
DFLAGGVAAAVpSK | ANT1 (ADP/ATP translocase 1) | H | S22 | 647.3 | 2+ | 847.4 | y9 | 165 | 12 | 8.1 |
H | 647.3 | 2+ | 263.1 | b2 | 165 | 20 | 6.8 | |||
H | 647.3 | 2+ | 235.1 | a2 | 165 | 36 | 9.7 | |||
| ||||||||||
YFPpTQALNFAFK | ANT1 (ADP/ATP translocase 1) | M, H | T84 | 767.9 | 2+ | 612.8 | y102+ | 215 | 20 | 0.1 |
M, H | 767.9 | 2+ | 563.8 | y10-H3PO42+ | 215 | 20 | 4.5 | |||
M, H | 767.9 | 2+ | 283.1 | a2 | 215 | 28 | 0.6 | |||
| ||||||||||
GpTGGALVLVLYDK | ANT4 (ADP/ATP translocase 4) | M, H | T293 | 697.4 | 2+ | 758.5 | y6 | 120 | 24 | 24.9 |
M, H | 697.4 | 2+ | 648.4 | M-H3PO4 | 120 | 12 | 29.1 | |||
M, H | 697.4 | 2+ | 326.1 | b5-H3PO4 | 120 | 20 | 37.8 | |||
| ||||||||||
pSVDEVNYWDK | BCKDHA (2-oxoisovalerate dehydrogenase subunit alpha) | M, H | S344 | 671.8 | 2+ | 1076.5 | y8 | 130 | 16 | 6.9 |
M, H | 671.8 | 2+ | 733.3 | y5 | 130 | 16 | 3.2 | |||
M, H | 671.8 | 2+ | 141.1 | a2-H3PO4 | 130 | 36 | 0.7 | |||
| ||||||||||
SGLGELILPENEPGSpSIMPGK | FH (Fumarate hydratase) | M, H | S363 | 1107.0 | 2+ | 720.3 | y6 | 250 | 40 | 37.8 |
M, H | 1107.0 | 2+ | 670.4 | b7 | 250 | 40 | 50.4 | |||
M, H | 1107.0 | 2+ | 309.2 | y3 | 250 | 40 | 62.4 | |||
| ||||||||||
MAGpSAWVSK | IDH3A (Isocitrate dehydrogenase [NADP], cytoplasmic) | M | S4 | 512.7 | 2+ | 795.4 | y8-H3PO4 | 130 | 16 | 9.4 |
M | 512.7 | 2+ | 724.4 | y7-H3PO4 | 130 | 12 | 6.2 | |||
M | 512.7 | 2+ | 242.2 | y2 | 130 | 24 | 21.7 | |||
| ||||||||||
SpSLATMAHAQSLVEAQPNVDK | OGDH (2-oxoglutarate dehydrogenase) | M | S103 | 762.4 | 3+ | 1050.5 | y20-H3PO42+ | 160 | 24 | 27.5 |
M | 762.4 | 3+ | 729.7 | M-H3PO43+ | 160 | 12 | 26.8 | |||
M | 762.4 | 3+ | 580.3 | y5 | 160 | 24 | 23.3 | |||
M | 762.4 | 3+ | 270.1 | b3-H3PO4 | 160 | 36 | 20.8 | |||
| ||||||||||
GpSLAAVAHAQSLVEAQPNVDK | OGDH (2-oxoglutarate dehydrogenase) | H | S103 | 731.7 | 3+ | 908.5 | y8 | 160 | 16 | 12.6 |
H | 731.7 | 3+ | 807.9 | y152+ | 160 | 8 | 14.7 | |||
H | 731.7 | 3+ | 580.3 | y5 | 160 | 20 | 14.3 | |||
| ||||||||||
VVNAPIFHVNSDDPEAVMpYVCK | OGDH (2-oxoglutarate dehydrogenase) | M, H | Y485 | 864.7 | 3+ | 1105.0 | y182+ | 190 | 20 | 35.3 |
M, H | 864.7 | 3+ | 592.8 | y92+ | 190 | 20 | 34.9 | |||
M, H | 864.7 | 3+ | 199.2 | b2 | 190 | 24 | 55.7 | |||
M, H | 864.7 | 3+ | 171.2 | a2 | 190 | 44 | 52.0 | |||
| ||||||||||
YHGHpSMSDPGVSYR | PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form) | M, H | S293 | 561.6 | 3+ | 498.2 | b82+ | 130 | 8 | 10.1 |
M, H | 561.6 | 3+ | 344.7 | y62+ | 130 | 12 | 9.7 | |||
M, H | 561.6 | 3+ | 301.1 | b2 | 130 | 24 | 7.9 | |||
M, H | 561.6 | 3+ | 282.6 | b5-H3PO42+ | 130 | 24 | 8.5 | |||
| ||||||||||
YHGHpSMSDPGVpSYR | PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form) | M, H | S293/S300 | 588.2 | 3+ | 670.4 | y6-H3PO4 | 130 | 20 | 10.4 |
M, H | 588.2 | 3+ | 573.3 | y5-H3PO4 | 130 | 24 | 7.9 | |||
M, H | 588.2 | 3+ | 384.7 | y62+ | 130 | 12 | 3.1 | |||
M, H | 588.2 | 3+ | 301.1 | b2 | 130 | 32 | 5.7 | |||
M, H | 588.2 | 3+ | 282.6 | b5-H3PO42+ | 130 | 24 | 11.2 | |||
M, H | 588.2 | 3+ | 174.6 | y22+ | 130 | 16 | 28.3 | |||
| ||||||||||
GGSMHMpYGK | PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form) | M | Y157 | 528.2 | 2+ | 586.2 | y4 | 130 | 20 | 19.0 |
M | 528.2 | 2+ | 455.2 | y3 | 130 | 20 | 29.4 | |||
M | 528.2 | 2+ | 212.1 | y2 | 130 | 32 | 3.0 | |||
| ||||||||||
INFDpSNSAYR | SUCLA2 (Succinyl-CoA ligase [ADP- forming], subunit beta) | M, H | S279 | 638.8 | 2+ | 804.4 | y7-H3PO4 | 130 | 24 | 15.7 |
M, H | 638.8 | 2+ | 506.3 | y4 | 130 | 24 | 13.0 | |||
M, H | 638.8 | 2+ | 228.2 | y2 | 130 | 16 | 10.9 | |||
M, H | 638.8 | 2+ | 86.1 | I immonium | 130 | 32 | 10.8 | |||
| ||||||||||
LTFDSSFpSPNTGK | VDAC1 (Voltage-dependent anion-selective channel protein 1) | M, H | S117 | 744.8 | 2+ | 740.4 | y7-H3PO4 | 215 | 28 | 8.6 |
M, H | 744.8 | 2+ | 524.3 | y5 | 215 | 28 | 7.6 | |||
M, H | 744.8 | 2+ | 215.1 | b2 | 215 | 24 | 7.0 | |||
M, H | 744.8 | 2+ | 120.1 | F immonium | 215 | 50 | 9.2 | |||
| ||||||||||
CTTDHISAAGPWLK | ACO2 (Aconitate hydratase) | M, H | Unmodified | 522.3 | 3+ | 837.5 | y8 | 130 | 12 | 5.9 |
M, H | 522.3 | 3+ | 608.4 | y5 | 130 | 16 | 7.5 | |||
M, H | 522.3 | 3+ | 155.8 | y1 | 130 | 32 | 7.7 | |||
| ||||||||||
AAYFGVYDTAK | ANT1 (ADP/ATP translocase 1) | M, H | Unmodified | 607.3 | 2+ | 1071.5 | y9 | 135 | 12 | 5.4 |
M, H | 607.3 | 2+ | 908.5 | y8 | 135 | 16 | 7.5 | |||
M, H | 607.3 | 2+ | 761.4 | y7 | 135 | 12 | 5.4 | |||
| ||||||||||
DFLAGGVAAAVSK | ANT1 (ADP/ATP translocase 1) | H | Unmodified | 607.3 | 2+ | 838.5 | y10 | 160 | 12 | 9.5 |
H | 607.3 | 2+ | 263.1 | b2 | 160 | 16 | 9.1 | |||
H | 607.3 | 2+ | 235.1 | a2 | 160 | 32 | 9.9 | |||
| ||||||||||
YFPTQALNFAFK | ANT1 (ADP/ATP translocase 1) | M, H | Unmodified | 727.9 | 2+ | 818.5 | y7 | 185 | 24 | 27.4 |
M, H | 727.9 | 2+ | 572.8 | y102+ | 185 | 20 | 25.2 | |||
M, H | 727.9 | 2+ | 283.1 | a2 | 185 | 24 | 23.0 | |||
| ||||||||||
GTGGALVLVLYDK | ANT4 (ADP/ATP translocase 4) | M, H | Unmodified | 657.4 | 2+ | 970.6 | y8 | 165 | 16 | 0.5 |
M, H | 657.4 | 2+ | 857.5 | y7 | 165 | 16 | 1.1 | |||
M, H | 657.4 | 2+ | 758.5 | y6 | 165 | 16 | 6.0 | |||
| ||||||||||
SVDEVNYWDK | BCKDHA (2-oxoisovalerate dehydrogenase subunit alpha) | M, H | Unmodified | 631.8 | 2+ | 1076.5 | y8 | 130 | 16 | 9.7 |
M, H | 631.8 | 2+ | 187.1 | b2 | 130 | 12 | 13.5 | |||
M, H | 631.8 | 2+ | 159.1 | a2 | 130 | 28 | 14.9 | |||
| ||||||||||
AMLDPEGILNPYK | D2HGDH (D-2-hydroxyglutarate dehydrogenase) | M | Unmodified | 734.9 | 2+ | 1038.6 | y9 | 160 | 20 | 15.4 |
M | 734.9 | 2+ | 203.1 | b2 | 160 | 24 | 14.0 | |||
M | 734.9 | 2+ | 175.1 | a2 | 160 | 32 | 14.1 | |||
| ||||||||||
GILNPYK | D2HGDH (D-2-hydroxyglutarate dehydrogenase) | M, H | Unmodified | 406.7 | 2+ | 642.4 | y5 | 100 | 4 | 16.3 |
M, H | 406.7 | 2+ | 415.2 | y3 | 100 | 12 | 11.0 | |||
M, H | 406.7 | 2+ | 171.1 | b2 | 100 | 4 | 6.9 | |||
| ||||||||||
SGLGELILPENEPGSSIMPGK | FH (Fumarate hydratase) | M, H | Unmodified | 1067.1 | 2+ | 1350.7 | y13 | 190 | 32 | 38.8 |
M, H | 1067.1 | 2+ | 670.4 | b7 | 190 | 36 | 33.4 | |||
M, H | 1067.1 | 2+ | 309.2 | y3 | 190 | 36 | 30.9 | |||
| ||||||||||
MAGSAWVSK | IDH3A (Isocitrate dehydrogenase [NADP], cytoplasmic) | M | Unmodified | 472.7 | 2+ | 813.5 | y8 | 100 | 8 | 10.6 |
M | 472.7 | 2+ | 742.4 | y7 | 100 | 8 | 20.9 | |||
M | 472.7 | 2+ | 175.1 | a2 | 100 | 12 | 21.6 | |||
| ||||||||||
GYDSDNPRVR | MUTA (Methylmalonyl-CoA mutase) | M, H | Unmodified | 594.8 | 2+ | 427.2 | y72+ | 130 | 8 | 22.5 |
M, H | 594.8 | 2+ | 336.1 | b3 | 130 | 28 | 18.8 | |||
M, H | 594.8 | 2+ | 185.1 | y1 | 130 | 32 | 20.4 | |||
| ||||||||||
SSLATMAHAQSLVEAQPNVDK | OGDH (2-oxoglutarate dehydrogenase) | M | Unmodified | 735.7 | 3+ | 908.5 | y8 | 160 | 16 | 5.7 |
M | 735.7 | 3+ | 779.4 | y7 | 160 | 16 | 9.6 | |||
M | 735.7 | 3+ | 580.3 | y5 | 160 | 20 | 7.9 | |||
| ||||||||||
GSLAAVAHAQSLVEAQPNVDK | OGDH (2-oxoglutarate dehydrogenase) | H | Unmodified | 705.0 | 3+ | 908.5 | y8 | 130 | 16 | 8.3 |
H | 705.0 | 3+ | 779.4 | y7 | 130 | 12 | 11.5 | |||
H | 705.0 | 3+ | 580.3 | y5 | 130 | 12 | 10.0 | |||
| ||||||||||
VVNAPIFHVNSDDPEAVMYVCK | OGDH (2-oxoglutarate dehydrogenase) | M, H | Unmodified | 838.0 | 3+ | 1157.5 | y202+ | 160 | 20 | 0.0 |
M, H | 838.0 | 3+ | 1065 | y182+ | 160 | 20 | 0.0 | |||
M, H | 838.0 | 3+ | 552.8 | y9 | 160 | 20 | 1.1 | |||
| ||||||||||
IEQLSPFPFDLLLK | OGDH (2-oxoglutarate dehydrogenase) | M, H | Unmodified | 834.5 | 2+ | 1297.8 | y11 | 160 | 28 | 3.1 |
M, H | 834.5 | 2+ | 1097.7 | y9 | 160 | 20 | 3.7 | |||
M, H | 834.5 | 2+ | 371.2 | b3 | 160 | 24 | 7.1 | |||
| ||||||||||
YHGHSMSDPGVSYR | PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form) | M, H | Unmodified | 534.9 | 3+ | 688.4 | y6 | 130 | 16 | 4.0 |
M, H | 534.9 | 3+ | 591.3 | y5 | 130 | 16 | 2.6 | |||
M, H | 534.9 | 3+ | 458.2 | b82+ | 130 | 8 | 4.4 | |||
| ||||||||||
GGSMHMYGK | PDHA1 (Pyruvate dehydrogenase E1 component subunit alpha, somatic form) | M | Unmodified | 488.2 | 2+ | 506.3 | y4 | 130 | 16 | 17.1 |
M | 488.2 | 2+ | 375.2 | y3 | 130 | 16 | 20.8 | |||
M | 488.2 | 2+ | 202.1 | b3 | 130 | 16 | 20.3 | |||
| ||||||||||
INFDSNSAYR | SUCLA2 (Succinyl-CoA ligase [ADP- forming], subunit beta) | M, H | Unmodified | 598.8 | 2+ | 969.5 | y8 | 130 | 16 | 9.2 |
M, H | 598.8 | 2+ | 822.4 | y7 | 130 | 20 | 8.2 | |||
M, H | 598.8 | 2+ | 228.2 | b2 | 130 | 16 | 10.4 | |||
| ||||||||||
LTFDSSFSPNTGK | VDAC1 (Voltage-dependent anion-selective channel protein 1) | M, H | Unmodified | 704.8 | 2+ | 845.4 | y8 | 180 | 20 | 1.8 |
M, H | 704.8 | 2+ | 524.3 | y5 | 180 | 28 | 0.9 | |||
M, H | 704.8 | 2+ | 215.1 | b2 | 180 | 20 | 0.9 |
All transitions verified for each listed peptide are shown. Amino acid residue numbering is based on the mouse homolog sequence in Uniprot.
Frag (V): fragmentor voltage
CE (V): collision energy
CV: coefficient of variation. Coefficients of variation were calculated from three repeated injections, with the exception of the ANT1 pT84 and OGDH unmodified Y485 peptides, which were based on duplicate injections.
M: the listed peptide corresponds to mouse protein sequence
H: the listed peptide corresponds to human protein sequence