Fig. 1.
M. synoviae LeuRS mischarges tRNALeu. (A) Leucylation by M. synoviae LeuRS was carried out by using 21 µM [14C]leucine (50 μCi/mL), 4 µM tRNALeu, and 100 nM enzyme. (B) Isoleucine mischarging by M. synoviae LeuRS incorporated 40 µM [3H]isoleucine (166 μCi/mL), 4 µM tRNALeu, and 1 µM enzyme. M. mobile LeuRS was used as a positive control. (C) Deacylation reactions for M. synoviae LeuRS contained ∼6.5 µM [3H]Ile-tRNALeu and 100 nM enzyme. E. coli LeuRS was used as the positive control. Abbreviations are as follows: ■, M. synoviae LeuRS (Ms); ▲, M. mobile LeuRS (Mm); ▼, E. coli LeuRS (Ec); and ◆, no enzyme control (noE). Error bars represented SDs calculated from triplicated reactions.