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. 2013 Feb 19;110(10):3817–3822. doi: 10.1073/pnas.1218374110

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Fig. 4. — Aminoacylation and misaminoacylation activities of M. mobile LeuRSs that contain hybrid CP1 domains. Aminoacylation of 4 μM tRNA^Leu in presence of (A) 21 μM [³H]leucine (318 μCi/mL), (B) 21 μM [³H]isoleucine (166 μCi/mL), (C) 20.2 μM [³⁵S]methionine (115 μCi/mL), and (D) 20 μM [¹⁴C]valine (259 μCi/mL). Symbols used are as follows: ■, MmLeuRS (Mm); ▲, MmLeuRS/CP1^Leu (Mm/CP1^Leu); ▼, MmLeuRS/CP1^Ile (Mm/CP1^Ile); and ◆, MmLeuRS/CP1^Val (Mm/CP1^Val). Error bars represent SDs from triplicated reactions.