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. 2013 Feb 19;110(10):3817–3822. doi: 10.1073/pnas.1218374110

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Fig. 5. — CP1 addition to hybrid M. mobile LeuRS enhances pretransfer editing. Reaction mixture contained 1 μM enzyme, 10 μM tRNA^Leu, or tRNA^Leu with A₇₆ replaced by dideoxyadenosine (ddA), 18.1 μM [α-³²P]ATP (40 μCi/mL), and 2.5 mM isoleucine. The AMP formation activities are measured for Mm LeuRS (A), MmLeuRS/CP1^Leu (Mm/ CP1^Leu) (B), MmLeuRS/CP1^Ile (Mm/CP1^Ile) (C), and MmLeuRS/CP1^Val (Mm/CP1^Val) (D). Symbols used are as follows: ◆, reaction without amino acid present (no AA); ▲, isoleucine (Ile); ▼, isoleucine with tRNA (Ile+tRNA); and ■, isoleucine with ddtRNA (Ile+ddA). Error bars represent the SD values based on three separate experiments.