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Mn binds to the noncanonical transition metal-binding site in CP. (A and B) ITC titrations and binding isotherms from integrated heat for CP ΔS1 are shown for the addition of (A) Zn2+ [Kd = 8.2 ± 1.5 (SD) nM] and (B) Mn2+ (no binding). (C and D) ITC titrations and binding isotherms from integrated heat for CP ΔS2 are shown for the addition of (C) Zn2+ [Kd = 3.4 ± 1.2 (SD) nM] and (D) Mn2+ [Kd = 5.8 ± 1.6 (SD) nM].
