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. 2013 Feb 19;110(10):E878–E887. doi: 10.1073/pnas.1219536110

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Fig. 5. — Pre–steady-state kinetic analysis of ribosome stalling. (A) A ribosome complex containing MDTS-peptidyl-tRNA in the P site and the UAA stop codon in the A site was reacted with RF1 to determine the rate of peptidyl-tRNA hydrolysis. Ala (gray) and Phe (black) mutants were characterized to test the contribution of each residue in the DTS motif. (B) A ribosome complex containing MEP-peptidyl-tRNA or derivatives was reacted with RF1. (C) Peptidyl-transfer rates for the E1 motif were obtained by reacting ribosome nascent chain complexes with excess ternary complex composed of Pro- or Phe-tRNA, EF-Tu, and GTP.