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. 2013 Feb 19;110(10):3800–3805. doi: 10.1073/pnas.1217358110

Fig. 4.

Fig. 4.

UBR4 loss results in multiple misregulations of autophagic induction and flux. (A) Immunoblotting of +/+ and UBR4−/− MEFs. (B) Immunoblotting of +/+ and UBR4−/− MEFs growing in normal or starvation medium for 90 min in the presence or absence of 0.2 μM bafilomycin A1 or 10 mM 3-methyladenine. (C) Immunostaining of transiently expressed LC3-GFP and endogenous ATG12 in +/+ and UBR4−/− MEFs cultured in normal or starvation medium for 90 min. (D) Time-course immunoblotting of +/+ and UBR4−/− MEFs following nutrient deprivation. Cells were cultured in normal or starvation medium for 90 min, followed by time-course immunoblotting of remaining proteins. (E) Pulse chase analysis of p62-V5 transiently expressed in HEK293 cells. (F) Quantitation of E.