Fig. 4.
Mutation of EspP residues 1066 and 1081 uncouples passenger domain translocation from the binding of the β domain to the Bam complex. AD202 transformed with pDULEBpa and pRI22 (Plac-espP) or a pRI22 derivative encoding EspP(G1066A) or EspP(G1081D) with an amber mutation at residue 1113 (A) or 1214 (B) were subjected to pulse-chase labeling after the addition of IPTG. One aliquot of cells was UV-irradiated and an equal aliquot was untreated, and PK was added to half of each sample. Immunoprecipitations were subsequently conducted using the indicated antisera. Only the UV-irradiated samples are shown; untreated samples are shown in Fig. S4.