Fig. 6.

Esp(G1066A) C-terminal passenger domain residues interact with SurA. AD202 transformed with pDULEBpa and pRI22 or a pRI22 derivative encoding EspP(G1066A) with an amber mutation at the indicated residue were pulse-labeled and subjected to a 1-min chase after the addition of IPTG. One aliquot of cells was UV-irradiated and an equal aliquot was untreated. Immunoprecipitations were subsequently conducted using anti-EspP C-terminal, anti-BamA, and anti-SurA antisera. Only the UV-irradiated samples are shown.