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. 2013 Feb 19;110(10):3812–3816. doi: 10.1073/pnas.1216691110

Fig. 3.

Fig. 3.

Effect of tetracyclines and glycylcyclines on tRNA selection. (A) Schematic illustrating the delivery of EF-Tu⋅tRNA⋅GTP ternary complex containing cognate Phe-tRNAPhe(Cy5-acp3U47) to 70S E. coli ribosomes containing P-site OH-tRNAfMet(Cy3-s4U8), leading to low (0.2) FRET during initial steps of codon recognition, and high (0.55) FRET upon A-site tRNA accommodation. Tigecycline is more effective than tetracycline at blocking the initial selection process. Tetracycline is nevertheless effective at preventing transitions into the fully accommodated, high-FRET state. (B–F) Single-molecule FRET imaging of aa-tRNA selection performed under direct 532-nm excitation following a 5-min incubation (B) in the absence of drugs or with (C) 40 µM tetracycline, (D) 40 µM tetracycline and 0.1 µM TetM, (E) 2 µM tigecycline, or (F) 2 µM tigecycline and 0.1 µM TetM.