Fig. 1.

Dynamic motions of apoSOD1^ΔIVΔVII measured by CPMG NMR. (A) The dynamic motions (red) are centered in the active-site sheet (β4 and β5) and the adjacent strand β6. (B) Representative data showing the CPMG amplitude decrease at high temperature. (C) Calculated chemical shift differences between the species undergoing dynamic exchange. (D) The population of the high-energy state decreases by protein destabilization by temperature, pH, and urea. (E) Sequence outline of apoSOD1^ΔIVΔVII showing the positions with dynamic exchange (red), the corresponding dynamics of apoSOD1^pwt (stars), and the GAG replacements of loops IV and VII.