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Comparison of in vitro furfural reductase activity and furfural resistance. NADH-linked furfural-dependent reductase activity (A) and furfural tolerance for growth (B) are shown for plasmid-free strains containing predicted optimal combinations of furfural resistance traits. Cell mass was measured from tube cultures (n = 3) grown for 48 h in AM1 minimal media containing 50 g/L xylose with 12.5 mM furfural. Data represent averages of at least two experiments with SDs.
