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. 2013 Feb 20;14(4):282–291.

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Fig 1 — PCR from amplified bFGF and SDS-PAGE of produced bFGF. A. The expected 459 bp product of bFGF amplified by PCR with primers that added restriction sites at both ends. M; Size marker, P1 and P2 bFGF PCR product bands, PD; bFGF PCR product digested with SacI/EcoRI, VD; pET28 vector digested with SacI/EcoRI, CP-; Colony PCR negative control, CP1-5; Colony PCR results for clones 1 to 5, VUd; Undigested pET28/bFGF vector, VDS; pET28/ bFGF vector digested with SacI, VDS/E and pET28/bFGF vector digested with SacI /EcoRI. B. SDS-PAGE results for different steps of bFGF production. Recombinant his-tagbFGF expressed and purified at a good concentration and purity. M; Protein size marker, L: Lysate, CO; Cut off, W1; Wash 1, W2; Wash 2, W3; Wash 3, E1; Elution 1 and E2; Elution 2.