Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 20;14(4):282–291.

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig 2 — Evaluation of human pluripotent stem cell colonies based on morphology seven days post-subculture (grades A-D). The quality of colonies was graded as follows: Grade A. (excellent) colonies had even morphology and well-defined edges. The cells in the colonies were dense and individual cells could not be easily distinguished. The colonies were thick, multilayered, homogenous, and exhibited 0-30% differentiation. The differentiated cells migrated and passed from the periphery of the colonies. Grade B. (good) with differentiation in 30-50% of the peripheral area. Grade C. (fair) colonies exhibited more than 50-80% differentiation. Grade D. (poor) colonies differentiated more than 80-100%, and exhibited a well differentiated morphology with inhomogeneous levels.