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. 2013 Feb 20;14(4):282–291.

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Fig 3 — Characterization of human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) expanded in the presence of bFGF. Lines are characterized after 15 to 18 passages. The cell lines retained key properties of pluripotent markers. Morphology of human embryonic (hESCs) and induced pluripotent stem cells (hiPSCs) and expressions of ALP, Oct4, Nanog, SSEA3, TRA-1-60, and TRA-1-81. Nuclei were stained with DAPI (blue). Dot plot diagram of flow cytometry shows co-expression of two markers. The karyotype of hESCs and hiPSCs after several passages with Royan-bFGF was normal.