Table 1.
Comparison of Royan bFGF with commercial bFGFs
| Treatment | Percent of human embryonic stem cell (hESC) grade A+B colonies. | ||||
|---|---|---|---|---|---|
| P1 | P2 | P3 | P4 | Average | |
| NTL | 60(1:3) | 70(1:2) | 70(1:3) | 65 | <0.05 |
| SML | 60(1:3) | 70(1:2) | 60(1:3) | 60 | |
| STML | 70(1:4) | 70(1:4) | 70(1:4) | 70 | 0.028 |
| STMF | 80(1:4) | 80(1:4) | 70(1:4) | 75 | |
| Sigma bFGF | 70(1:4) | 70(1:4) | 70(1:4) | 70 | 0.566 |
| Invitrogen bFGF | 70(1:4) | 60(1:4) | 70(1:4) | 65 | |
All groups contain some treatment that the each treatment is shown in a capital letter S; Sonicated, N; Not sonicated, T; Tween 20, M: β-mercaptoethanol, L; Lyophilized and F; Fresh bFGF. NTL; Not treated with sonicator in the purification process. NTL contains Tween 20 in storage buffer and was lyophilized for long-term storage. STMF group: Treated with sonication, contained Tween 20 and β-mercaptoethanol in storage buffer and maintained at 4℃ without lyophilization. The percent of colonies that were grades A or B (A+B, see Fig 2) during each passage, P1-4: Passages 1-4, (1:n): Indicates proliferation rate during each passage. Clearly, the STMF group is the best group because it has highest percentage of undifferentiated cells and proliferation rate.