Table 1.
The sequences, annealing temperatures, and product sizes of the primers used to amplify genes of interest
| Genes | Product size (bp) | Annealing temperature (˚C) | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|---|
| Oct4-A | 496 | 63.5 | CTTCTCGCCCCCTCCAGGT | AAATAGAACCCCCAGGGTGAGC |
| Oct4-B | 267 | 64 | AGACTATTCCTTGGGGCCACAC | CTCAAAGCGGCAGATGGTCG |
| Oct4-B1 | 492 | 64 | AGACTATTCCTTGGGGCCACAC | CTCAAAGCGGCAGATGGTCG |
| Sox2 | 426 | 60 | ATGGGTTCGGTGGTCAAGTC | GTGGATGGGATTGGTGTTCTC |
| Nanog | 470 | 59 | ACCTATGCCTGTGATTTGTGG | AAGAGTAGAGGCTGGGGTAGG |
| Nucleostemin | 747 | 60 | CAGAGATCCTCTTGGTTGCAG | AATGAGGCACCTGTCCACTC |
| ZFX | 465 | 60 | TGATTCCAGGCAGTACCAAAC | TGACGAAAACCCTTACCACAC |
| CD31 | 578 | 59.5 | AGGACATCCATGTTCCGAGA | TGAACCGTGTCTTCAGGTTG |
| CD34 | 219 | 56 | TGAGCCTCTCACCTGTACTC | AGGAGCTGATCTGGGCTATG |
| KDR | 180 | 61 | CCCTGCCGTGTTGAAGAGTT | GGACAGGGGGAAGAACAAAA |
| VCAM1 | 375 | 58 | AGAAATGCCCATCTATGTCC | CGGCATCTTTACAAAACCTG |
| Tie1 | 173 | 57 | GCAAACTCTGCTGTCTAACC | GGATGCCCAGGATAGCTATG |
| GAPDH (1) | 139 | 64 | GCCCCAGCAAGAGCACAAGA | TAGGCCCCTCCCCTCTTCAA |
| GAPDH (2) | 224 | 60 | AGGGTCTCTCTCTTCCTCTTGTGCTC | CCAGGTGGTCTCCTCTGACTTCAACA |