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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 6;190(6):2984–2993. doi: 10.4049/jimmunol.1201650

Figure 1. Host cell-derived MIF promotes B16 melanoma outgrowth and reduces pro-inflammatory macrophage phenotypes in mice.

Figure 1

(A) MIF-deficiency impairs in vivo tumor growth in B16 transplanted mice. C57BL/6 MIF+/+ and MIF−/− mice were challenged with B16 cells s.c. and tumor volumes were plotted. The data represent the average tumor volumes of 8 mice/group ± SEM representative of three independent experiments. (B) Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. (C, D) Resident PECs from MIF+/+ and MIF−/− C57BL/6 mice bearing a s.c. melanoma tumor (n = 10) were pooled and activated in vitro with (C) LPS alone or (D) either LPS and DMSO (vehicle control) or LPS and 4-IPP (50 μM) for 24 hours. mRNA and protein expression was analyzed by qPCR and ELISA. Nitric oxide levels were measured using Greiss reagent. Data represents the average ± SEM of duplicate samples and are representative of three independent experiments. P values = *, p≤0.05; **, p≤0.005; ***, p≤0.0005.