Table 1. Cardiovascular Parameters.
The hemodynamic and anatomic responses to 1 week Ang-II infusion (discussion in text). Baseline values (before treatment) and experimental values (after 1 week) are expressed as mean ± SEM. The % change was calculated within the same mouse with parameters measured before and after Ang-II infusion. BW: body weight, HR: heart rate, LVEDD and LVESD: left ventricular end diastolic/systolic diameter, LVEDV and LVESV: left ventricular end diastolic/systolic volume, LVPW and LVAW: left ventricular posterior/anterior wall thickness, -d/-s: during diastole/systole, E-lin Dec Time: E-linear deceleration time, Peak Vel: peak velocity, EjT: ejection time, Tei Index (= myocardial performance index) was calculated as [(Isovolumetric Relaxation Time + Isovolumetric Contraction Time)/Ejection Time] and is heart rate independent. SBP: systolic blood pressure.
| C57 | C57 | C57 | TNFR1-KO | TNFR1-KO | TNFR1- KO | TNFR2- KO | TNFR2-KO | TNFR2- KO | |
|---|---|---|---|---|---|---|---|---|---|
| baseline | 1 week | % change | baseline | 1 week | % change | baseline | 1 week | % change | |
| Global Parameters | |||||||||
| n | 6 | 6 | 6 | 8 | 8 | 8 | 8 | 8 | 8 |
| BW, g | 26.6±0.6 | 24.8±0.5 # | 7±1 | 24.0±0.9 | 23.0±0.8 | 4±1 | 22.5±0.7 | 21.1±0.9 | 7±2 |
| HR, bpm | 446±21 | 483±21 | 9±6 | 474±25 | 516±17 | 10±6 | 506±17 | 495±20 | 1±5 |
|
| |||||||||
| Cardiac Echocardiographic Parameters | |||||||||
| LVEDD, mm | 3.76±0.12 | 3.81±0.18 | 2±7 | 3.64±0.13 | 3.47±0.17 | −5±3 | 3.66±0.08 | 3.29±0.08 # | −10±3 |
| LVEDV, mm3 | 68.4±4.6 | 63.4±7.2 | −3±15 | 63.2±5.1 | 51.1±5.9 # | −20±6 | 61.3±2.9 | 44.1±2.5 # | −27±6 |
| LVESD, mm | 2.76±0.13 | 2.75±0.24 | 2±10 | 2.66±0.11 | 2.71±0.17 | 2±5 | 2.65±0.09 | 2.54±0.13 | −2±8 |
| LVESV, mm3 | 27.6±2.6 | 30.1±6.8 | 18±31 | 24.3±3.1 | 28.6±4.4 | 19±13 | 24.7±2.3 | 24.1±2.9 | 10±21 |
| Fractional Shortening | 33.1±1.6 | 29.7±1.3 | −9±6 | 33.3±1.5 | 31.2±1.7 | −4±8 | 31.9±1.5 | 31.6±1.4 | 1±6 |
| Ejection Fraction | 62.7±1.9 | 55.7±2.3 | −10±5 | 62.4±2.1 | 59.6±2.6 | −3±6 | 60.4±2.2 | 60.5±2.1 | 1±5 |
| LVPW-d, mm | 0.78±0.08 | 1.21±0.09 # | 58±6 | 0.78±0.06 | 0.79±0.04 | 4±7 *† | 0.73±0.04 | 1.00±0.07 # | 37±5 |
| LVPW-s, mm | 1.00±0.09 | 1.38±0.08 # | 44±12 | 0.92±0.07 | 0.95±0.04 | 8±9 * | 0.96±0.04 | 1.19±0.08 # | 24±9 |
| LVAW-d, mm | 0.59±0.03 | 0.86±0.05 # | 48±12 | 0.69±0.06 | 0.67±0.05 | 0±9 *† | 0.57±0.03 | 0.78±0.04 # | 40±11 |
| LVAW-s, mm | 0.79±0.07 | 1.15±0.09 | 59±29 | 0.76±0.07 | 0.99±0.06 # | 36±11 | 0.72±0.05 | 1.07±0.06 # | 55±14 |
| LV Mass (calc), mg | 88.0±7.9 | 138.9±7.2 # | 63±12 | 88.4±7.5 | 85.3±8.1 | −1±10 *† | 76.8±4.9 | 99.6±6.8 # | 33±10 |
|
| |||||||||
| Cardiac Doppler Parameters | |||||||||
| E-lin Dec Time, ms | 49.9±7.3 | 20.2±1.5 # | 56±5 | 39.2±5.9 | 36.5±5.7 | 2±18 * | 56.0±4.5 | 40.9±5.3 # | 25±9 |
| E-Peak Vel, cm/s | 75.2±2.7 | 81.9±5.0 | 10±9 | 70.9±1.3 | 76.4±3.6 | 8±5 | 78.8±2.1 | 71.6±4.7 | 9±5 |
| Peak Vel, cm/s | 109±7 | 107±11 | 2±8 | 105±6 | 110±8 | 5±5 | 125±8 | 104±6 | 16±6 |
| Pre-EjT, ms | 15.7±1.1 | 17.7±0.4 | 15±9 | 17.9±0.5 | 16.1±0.5 | 10±3 *† | 15.4±0.3 | 18.0±0.5 # | 18±5 |
| Pre-EjT/EjT | 0.32±0.03 | 0.38±0.02 | 22±9 | 0.36±0.01 | 0.35±0.02 | 3±4 *† | 0.33±0.01 | 0.41±0.01 # | 26±4 |
| Tei Index | 0.61±0.07 | 0.70±0.04 | 17±7 | 0.68±0.02 | 0.65±0.03 | 4±6 *† | 0.67±0.03 | 0.78±0.03 # | 18±4 |
| Blood Pressure Parameters | |||||||||
| SBP, mm Hg | 117±5 | 155±4 # | 34±8 | 115±2 | 126±4 | 9±4 *† | 120±3 | 151±4 # | 25±5 |
P< 0.05 between baseline and 1 week of corresponding group
P< 0.05 between TNFR1-KO and C57
P< 0.05 between TNFR1-KO and TNFR2-KO