Figure 3.
VH–DJH rearrangements upon conditional Pax5 activation in thymocytes. Pre-T cells were isolated by FACS sorting as DP thymocytes from Ikneo/+ mice carrying the lck-cre transgene followed by PCR quantification of DH–JH and VH–DJH rearrangements as described in Figure 1B. Ikneo/+ pre-T cells and stromal ST2 cells served as negative controls and Pax5+/+ pro-B cells as positive control for the detection of VH–DJH rearrangements. Cre-mediated conversion of the Ikneo to the IkPax5 allele was determined by PCR (bottom row).