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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: J Mol Cell Cardiol. 2009 Jan 27;47(1):5–14. doi: 10.1016/j.yjmcc.2009.01.008

Fig. 8.

Fig. 8

miR-21 regulates AP-1 activity via its target gene PDCD4. Activator protein 1 (AP-1) was measured using luciferase assay. Cultured cardiac myocytes pretreated with vehicle, control oligos (LNA-scramble and pre-scramble), LNA-anti-miR-21 (30 nM), pre-miR-21 (30 nM), ad-GFP (30 MOI), Ad-PDCD4 (30 MOI), Ad-PDCD4-Lack (30 MOI), or pre-miR-21 plus Ad-PDCD4-Lack for 4 h, were transfected with Ad-AP1-Luc for 5 h with 10 PFU/cell. Luciferase activity was measured after 24 h. (A) Luciferase activity was decreased by overexpression of PDCD4 via either Ad-PDCD4 or AD-PDCD4-Lack. (B) LNA-anti-miR-21 decreased luciferase activity. In contrast, the luciferase activity was increased by pre-miR-21; however, the pre-miR-21-induced increase in luciferase activity was totally blocked after transfection of adenovirus expressing PDCD4 without the miR-21 binding site (Ad-PDCD4-Lack). Note: n=5; *P<0.05 compared with vehicle control. #P<0.05 compared with pre-miR-21 group.