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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Exp Cell Res. 2012 Dec 5;319(6):875–887. doi: 10.1016/j.yexcr.2012.11.019

Figure 5.

Figure 5

Effects of combined treatment of small molecular inhibitors and sodium arsenite on cell cycle and apoptosis of human NSC. (A, B) Cell cycle-apoptosis analysis was performed after treatment of human NSC with indicated inhibitors alone or in combination with 2 μM sodium arsenite. Pan-caspase inhibitor zVAD-fmk (40 μM) and caspase-9 inhibitor LEHD (40 μM) were also used. PI staining of DNA and FACS analysis was performed 24 h after indicated treatment. A typical experiment is presented in panel A; pooled results of four independent experiments are shown in panel B. Error bars represent means ± S.D. (p < 0.05, Student’s t test). (C) Western blot analysis of PARP-1 cleavage after indicated treatments of human NSC. Beta-Actin was used as a loading control. (D) Self-renewal of human NSC in the absence (control) and in the presence of sodium arsenite (2 μM) or in combinations of LY294002 (40 μM) and sodium arsenite (2 μM); BMS345541 (10 μM) and sodium arsenite (2 μM). Confocal analysis of immunofluorescent image of human NCS was performed using monoclonal antibody against Nestin (red) and polyclonal antibody against Sox2 (green). Relative cell survival is indicated.