Table 2.

Primers used for qRT-PCR experiments

Gene	Function	Locus ID	Primer sequence (forward and reverse)
betB	betaine aldehyde dehydrogenase	SACOL2628	5′-AATTGCTGTTGGTGGTAAACG-3′
			5′-TAACGACAGGTCCGAAAACC-3′
dnaK	DnaK protein	SACOL1637	5′-CCGGTGACAACAAACTTGG-3′
			5′-TCAGCAGCATCTTTCAAACG-3′
mmpL	MmpL efflux pump, putative	SACOL2566	5′-GGAATGACATCTACAGAAGTAGGC-3′
			5′-AACTGCTAGTCCAATCATTACGG-3′
purA	adenylosuccinate synthase	SACOL0018	5′-GAGGTTGGTCGTGAATACGG-3′
			5′-TGGGTACTCAGTAATTTCTTTACCG-3′
purM	phosphoribosylaminoimidazole synthetase	SACOL1080	5′-AATATGGGTATTGGCTATACGG-3′
			5′-CACAATATGACCAATTTGATAGGC-3′
rpmI	ribosomal protein L35	SACOL1726	5′-TGCCAAAAATGAAAACTCACC-3′
			5′-GAGATGTGAAAGCTCTTGAACG-3′
tenA	transcriptional regulator, TenA family	SACOL2086	5′-TAGGAGCTGACGCATTACGC-3′
			5′-CCCATTGTTCTAGTGTCATAGCC-3′
vraR	DNA-binding response regulator VraR	SACOL1942	5′-AAAGAAGCAATTGCCAAAGC-3′
			5′-TGAGTCGTCGCTTCTACACC-3′
vraS	histidine kinase sensor	SACOL1943	5′-AGTGCCGATGAAAGTTGTGC-3′
			5′-TTTTGTACCGTTTGAATGACG-3′
vraX	VraX protein	SACOL0625	5′-TCGACAGTATCACCATGAAGG-3′
			5′-TTTCAGTATCACTAAATGAATCGTCAC-3′