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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Traffic. 2013 Jan 24;14(4):382–398. doi: 10.1111/tra.12038

Figure 6. Selective protein secretion in liver cells over-expressing nuclear CREB-HΔTMC.

Figure 6

Parental HepG2 cells (H) and HepG2 cells selected for the constitutive expression of CREB-H-ΔTMC (H-CH), were analysed. (a) H-CH cells express the doublet form of CREB-H-ΔTMC identical to that seen with 293Tet-on cells which localized exclusively to nuclei (b). Scale bar, 10 μM. (c) Total cell samples from H or H-CH cells, along with secreted proteins from the corresponding media, were analysed by total protein staining on 10% (lanes 1–4) or 15% (lanes 5,6) SDS PAGE gels. No differences in protein levels could be discerned at this level in the total cell samples. Several proteins, including albumin, remained unchanged in the profile of secreted proteins; however, a number of novel species, including two prominent bands were observed in the medium of H-CH cells. Bands for which unequivocal identifications were made included albumin, Apo A-IV, Apo AI and sPLA2, as indicated.