Figure 2.

Defining the phenotypic proximity of M regs to macrophages in other states of activation. (a) M regs and a panel of nine comparator cell types were generated from fluorescence-activated cell sorting (FACS)-sorted CD11b⁺ Ly6C⁺ Ly6G^– B6 bone marrow monocytes. Three series of comparator cell types were generated in fully independent experiments. (b) Hierarchical clustering (Manhattan; average linked) of the reporter sets returned by one-way ANOVA which were significantly and highly differentially regulated in any two of the comparator macrophage populations. Red shading indicates a higher expression of a certain reporter in the respective sample compared to the median of all samples, a green shading indicates downregulation. (c) Confirmation by flow cytometry of the relative upregulation of podoplanin, CD127, and CD301 (MGL1) cell-surface expression in B6 M regs, but not other macrophage types. Open traces represent specific signals and shaded traces represent fluorescence-minus-one (FMO) isotype controls. Data are representative of three independent experiments.