Figure 3.

Exposure to human serum influences the development of mouse M regs. Mouse M regs are generated in the presence of human serum because development of human M regs depends upon factors present in human serum and not in fetal calf serum (FCS). (a) Hierarchically clustered heatmap (Pearson uncentered, average linked) depicting reporters returned by significance analysis of microarrays (SAM) (estimated FDR = 0) as significantly upregulated in M regs in comparison to interferon-γ (IFN-γ) MΦ. Three series of comparator cell types were generated in fully independent experiments. (b) Confirmation by flow cytometry of the relative upregulation of CD200, CD138, and CD38 cell-surface expression in B6 M regs. Open traces represent specific signals and shaded traces represent fluorescence-minus-one (FMO) isotype controls. Data are representative of three independent experiments.