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. 2013 Jan 8;21(2):423–432. doi: 10.1038/mt.2012.232

Figure 4.

Figure 4

Detection of human MnSOD expression and ROS levels in BM cell compartments of MnSOD-PL–treated mice. (a) Expression of human MnSOD mRNAs in different BM cell fractions from the MnSOD-PL– or vector-PL–treated mice measured by RT-PCR. Lanes 1, 3, and 5 represent the samples from the MnSOD-PL–treated mice; lanes 2, 4, and 6 represent the samples from the vector-PL–treated mice. The stromal cells were sorted CD45− adherent cells. (b) ROS levels in MnSOD-PL– or vector-PL–treated BM cells detected by flow cytometry. BM cells were harvested 36 hours after MnSOD-PL treatment and 12 hours after TBI. *P < 0.05; **P < 0.01. BM, bone marrow; DCF-DA, 2′,7′-dichlorofluorescene diacetate; MnSOD-PL, manganese superoxide dismutase-plasmid and lipofectin complex; ROS, reactive oxygen species; RT-PCR, reverse transcription-PCR; TBI, total body irradiation.