Figure 5.

Duration of siCAG response. (a) Slot-blot hybridization assay to determine the levels of CUG^exp accumulation in tibialis anterior muscle. Muscle was harvested 7 or 14 days after electroporating 3 µg of siCAG. Hybridization signal from (CAG)₈ probe (CUG^exp RNA) was normalized to mouse skeletal actin probe. Each bar represents the mean ± SD for indicated number of mice and bases on two independent hybridization experiments. *P < 0.0001, **P < 0.05 for siRNA versus untreated muscle (Student's t-test). (b) RT-PCR splicing assay for five MBNL1-dependent exons. Analyses were performed 7 or 14 days after electroporating 3 µg of siCAG (si) or saline (sal).