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. Author manuscript; available in PMC: 2013 Mar 11.
Published in final edited form as: Cell Rep. 2013 Feb 14;3(2):301–308. doi: 10.1016/j.celrep.2013.01.021

Figure 2. Impact of TAF15 knockdown on the transcriptome of mouse neurons.

Figure 2

A, B. TAF15 mRNA (A) and protein levels (B) after siRNA knockdown of TAF15 (KD), compared to control knockdown (CTRL), in mouse neurons. Quantitations (A, B) are shown as mean ± s.d., from three biological replicates; changes are significant (P<0.01).

C, D. Scatter plot of genes (C) and exons (D) expression calculated by RNA-Seq from 3 KD and 3 CTRL samples. Differentially expressed genes and exons (N=3; P<0.01) are highlighted, glog; generalized logarithm base 2.

E. RT-PCR validations of differentially included exons in mRNAs whose levels did not change upon TAF15 knockdown. Shown are schematics (constitutive exon: black box; differentially included exon: red box; intron: black line) with primers (arrows) and position of TAF15-peaks (blue boxes). Quantitation (error bars; s.d) and representative gels from RT-PCRs are shown. All detected changes are significant (P<0.01).

See also Figures S2 and S3.