Skip to main content
. Author manuscript; available in PMC: 2014 Mar 28.
Published in final edited form as: J Control Release. 2013 Jan 7;166(3):227–233. doi: 10.1016/j.jconrel.2012.12.029

Fig. 1.

Fig. 1

Evaluating transfection efficiency of nanoparticle mRNA and naked mRNA in vitro. (A) Aggregation kinetics of p/mLuc in 0-100% mouse serum. (B) In vitro p/mGFP and n/mGFP transfection of primary DCs and DC cell lines. (C), (D) In vitro p/mLuc transgene expression kinetics of primary human peripheral blood monocyte-derived DCs and primary mouse bone marrow precursor-derived DCs respectively (‘X’ represents background luminescence). Cells seeded on the same 96-well plate were transfected at various time points with 0.4 μg mLuc (n=5) and assayed after 15 hours. Results were averages of 3 independent experiments.