Figure 3.
Bacterial colonization in mice infected with Y. pestis EV76 plus iron. Mice were infected i.v. or i.n. with sublethal doses of Y. pestis EV76 as described in Fig 2. The blood, spleen, liver and lungs were collected, homogenized, and plated (in serial dilutions) on agar CIN base plates, and the plates were incubated to determine the number of colonizing organisms. The results are expressed as individual CFU/g of organ or ml of blood. Horizontal lines indicate the mean bacterial counts; the dotted line shows detection limit.