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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Comp Immunol Microbiol Infect Dis. 2012 Nov 26;36(2):113–128. doi: 10.1016/j.cimid.2012.10.005

Figure 4.

Figure 4

Representative histological analysis of the spleen, liver and lungs from mice infected with Y. pestis EV76 plus iron as described in Fig. 2. Mice treated with iron i.p. or i.n. were included as controls. Histological sections from spleen (A and B), liver (C and D), and lungs (E-J) were fixed, processed and stained with H&E to visualize tissue architecture on days 2 and 4 for i.n. and i.v.-infected mice, respectively. Mice infected i.v. with Y. pestis EV76 exhibited necrotic areas in the spleen (B), a microabscess in the liver (D), and very early pneumonia in the lung (F) and are indicated with arrowheads (B and D) and arrows (F). Mice infected i.n. showed signs of bronchopneumonia (H and higher magnification in J) with a massive neutrophil infiltration in the lungs (J, insert). Mice that received only iron i.n. had a mild transient peribronchial inflammation consistent with chemical injury (G, higher magnification in J). Magnifications: 20× (A-D, G and H), 40× (E, F, I and J) and 60× (J insert).