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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 15;190(6):2913–2923. doi: 10.4049/jimmunol.1202670

Figure 1. Exposure to intraamniotic LPS induces MnSOD expression and activity in newborn rat lung.

Figure 1

A) Total pulmonary SOD activity measured in whole lung homogenate from newborn rats exposed to intraamniotic LPS. Saline, intraamniotic saline injection; LPS, intraamniotic LPS injection. *, p<0.05 vs. saline control. Values are means ± SE, n=4 animals/group.

B) Representative Western blot showing SOD1, MnSOD, and SOD3 in whole lung homogenate from newborn rats following exposure to intraamniotic saline vs. LPS. β-actin is shown as a loading control.

C) Densitometric evaluation of SOD1, MnSOD, and SOD3 protein expression in whole lung homogenate from newborn rats exposed to intraamniotic saline vs. LPS. *, p<0.05 vs. saline control. Values are means ± SE, n=4 animals/group.

D) Representative immunofluorescence and H&E staining of newborn rat lungs following intraamniotic injection of saline (A–D) or LPS (E–H), all at 200X magnification. MnSOD was stained in red, vWF in green, and DAPI in blue. LPS, lipopolysaccharide; vWF, von Willebrand factor; MnSOD, manganese superoxide dismustase; DAPI, 4',6-diamidino-2-phenylindole.