Figure 5.

Dietary GSPs improve the ability of DCs to induce CHS responses in wild-type mice, but not in XPA-KO mice. Donor mice (wild-type and XPA-KO) were UVB-irradiated, sensitized with DNFB 24 hours after the last UVB exposure. Mice were sacrificed 24 hours after sensitization, and lymph nodes were harvested, single cell suspension was made, and CD11c⁺ cells were positively selected using MACS system. Syngeneic recipient mice were injected subcutaneously with 5× 10⁵ CD11c⁺ cells obtained from donor mice. Recipient mice were ear challenged with DNFB 5 days after injection of cells, and ear skin thickness was measured before and 24 and 48 hours after challenge. The change in ear thickness is reported as the mean of millimeters (x 10⁻²) ± SD, n=5 per group. ^*Significantly greater CHS response versus recipient of CD11c+ from UVB+ DNFB treated mice, P<0.001; ^¶Significantly lower CHS response versus the positive control (DNFB-sensitized) group, P<0.001.