Table 1.

Effect of co-culture of dendritic cells from wild-type and XPA-KO mice with CD4+ T cells on cytokine production

Cytokines	Wild-type			XPA-KO
	Control	UV	GSPs +UV	Control	UV	GSPs+ UV
IFNγ	320	152 (53)	353 (132)*	212	131 (38)	152
IL-4	215	296 (38)	197 (33)*	208	328 (57)	250
IL-10	456	844 (85)	659 (22)*	308	532 (73)	487

Dendritic cells were isolated from the draining lymph nodes of different treatment groups, as described in Materials and Methods. CD4⁺ T cells isolated from the spleens of naïve wild-type mice were labeled with CFSE and co-cultured with CD11c+ cells in the ratio of 10:1 for 4 days. Cells were harvested, and cell supernatants were subjected to the analysis of cytokines by ELISA. Values in parentheses under UV-exposed groups indicate percent reduction (IFNγ) or percent increase (IL-4, IL-10) in the amount of cytokines secreted by CD4+ T cells compared to controls. Asterisks (*) indicate that co-culture of DCs from GSPs-treated wild-type mice with CD4+ T cells enhanced the production of IFNγ (132%) and reduced the production of IL-4 (33%) and IL-10 (22%) compared to non-GSPs-treated UVB-irradiated mice in wild-type group. Cytokine concentration is reported in terms of mean values and pg/2 million cells, n=2. Standard deviation to mean values of cytokine content was in the range of 4-8% in each group.