Predicted structure of RBPL-1 (TAG-214). The conserved domains DWNN, zinc knuckle, and RING finger are indicated by blue, red, and gray boxes, respectively. The nuclear localization signal (NLS) is indicated by a green box. b. Protein sequence alignment highlights the conservation of the DWNN, Zn knuckle, and RING finger domains among human, rat, and C. elegans RBBP6 proteins. c. Full-length rbpl-1 mRNA cloned by RT-PCR. d. RBPL-1 expressed in E. coli. Upper: SDS-PAGE analysis of the bacterially expressed GST-RBPL-1 fusion protein, indicated by the red arrow. Lower: western blotting analysis of GST-RBPL-1 fusion protein (the red arrow) using an anti-GST antibody.