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. 2013 Mar 11;8(3):e58656. doi: 10.1371/journal.pone.0058656

Figure 3. Differences in quantity of ageing biomarker proteins in individual Ae. aegypti females at various adult ages.

Figure 3

Semi-quantitative western analysis was performed using polyclonal antibodies against the four ageing biomarker candidates; A. ADF, B. eIF5A, C. cuticle protein Q17LN8 and D. AFP. Below each panel is the corresponding Direct Blue 71-stained total-protein image from each membrane indicating consistent sample loading. E. Relative protein quantities determined by standardizing Western analysis bands (shown by arrows in A to D) by total protein and a gel standard sample. Values indicate the mean±SD standardized pixel volumes from five individual females at each age.