Figure 4. Inhibition by steviol of forskolin-stimulated apical chloride current.

Under permeabilization conditions, MDCK cell monolayers were mounted in hemichambers filled with a chloride gradient buffer. (A) Representative currents of acute effect. Steviol at all doses was added to both apical and basolateral hemichambers after stimulation of the chloride current by forskolin (post-treatment) and the currents were recorded at 50, 100, and 200 µM of steviol. At the end of experiment, 50 µM of GlyH-101 was added. (B) Summary of the data for the acute effect of steviol on apical chloride current in basolaterally permeabilized MDCK cell monolayers. (4 separate experiments, mean±SE; n = 4; **P<0.01 compared with controls)