Figure 5. Inhibition by steviol of short-circuit current measurement in dose- and time-dependent manners.

MDCK cell monolayer treated with steviol at the indicated concentrations or vehicle for 24 h were mounted in hemichambers filled with Krebs' buffer solution. After stimulation of current by forskolin, steviol inhibition was seen to have inhibited I_sc in a dose-dependent fashion. Representative tracings (A) and the summary of the data of 3–10 separate experiments (B) are shown (mean±SE; n = 3−10; **P<0.01 compared with controls). (C) Representative time-response tracings of short-circuit current measurements. Monolayers were pretreated with 100 µM of steviol for 5 minutes and 2, 6, 12, and 24 h. (D) Summary of the data from time-response experiments. (mean±SE; n = 7−16 separate experiments; **P<0.01 compared with controls)