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. 2013 Mar 11;8(3):e58871. doi: 10.1371/journal.pone.0058871

Figure 6. Steviol inhibits forskolin-stimulated apical chloride current in a time-dependent manner.

Figure 6

After permeabilization of the basolateral membrane with amphotericin B, MDCK cell monolayers were mounted in hemichambers filled with a chloride gradient buffer. 20 µM of forskolin was added to stimulate apical chloride current. (A) Representative tracings of chronic effect (pretreatment with steviol for 5 minutes, 2, 6, 12, and 24 h) were shown. At the end of the experiment, 50 µM of Gly-H101 was added. (B) Summary of the data for the chronic effect of steviol on apical chloride current in permeabilized MDCK cell monolayers (mean±SE; n = 5−11 separate experiments; **P<0.01 compared with controls)