Figure 4. Staining of cytology specimens using EGFR mutation-specific antibodies.

The total EGFR protein in cytology samples could be stained with EGFR (D38B1) antibody (A, D and G, 400×). Samples without EGFR mutations were not stained with two mutation-specific antibodies (B and C, 400×). Sample with E746–A750 deletion mutation were stained with E746–A750 deletion (6B6) specific antibody (E, 400×) and samples with L858R mutation were stained with L858R mutant (43B2) specific antibody (I, 400×).