Table 1. Xenopus F box proteins were screened for Ku sensitivity.

F-box protein	IMAGE number	Xenopus species	Expressed in RL	DNA binding	DSB- dep	Ku-dep
Ccnf	5049128	laevis	+	–	N/A	N/A
Fbh1	6316796	laevis	+	+	–	N/A
Fbxl12	7682433	tropicalis	+	+	+	+
Fbxl14	3402730	laevis	–	N/A	N/A	N/A
Fbxl16	7660186	laevis	+	–	N/A	N/A
Fbxl17	6636605	laevis	+	–	N/A	N/A
Fbxl19	6864660	laevis	+	+	–	N/A
Fbxl20	5543149	laevis	–	N/A	N/A	N/A
Fbxl22	8318195	laevis	+	–	N/A	N/A
Fbxl3	5440173	laevis	+	–	N/A	N/A
Fbxl5	6317694	laevis	+	–	N/A	N/A
Fbxl8	7765941	laevis	+	–	N/A	N/A
Fbxo11	6644003	laevis	+	–	N/A	N/A
Fbxo15	7856627	tropicalis	+	–	N/A	N/A
Fbxo24	7708113	tropicalis	+	+	ND	ND
Fbxo25	6319137	laevis	+	–	N/A	N/A
Fbxo27	7011501	laevis	+	–	N/A	N/A
Fbxo28	7601995	tropicalis	+	+	–	N/A
Fbxo30	5384657	tropicalis	+	–	N/A	N/A
Fbxo36	5307822	tropicalis	+	–	N/A	N/A
Fbxo38	7391082	laevis	+	–	N/A	N/A
Fbxo41	7692156	tropicalis	+	–	N/A	N/A
Fbxo42	6953888	laevis	+	–	N/A	N/A
Fbxo44	4959662	laevis	+	–	N/A	N/A
Fbxo5-A	6323527	laevis	+	–	N/A	N/A
Fbxo9	4202748	laevis	+	–	N/A	N/A
Fbxw5	6980943	tropicalis	+	–	N/A	N/A
Kdm2b	6862732	laevis	+	+	–	N/A
Skp2	7393117	laevis	+	–	N/A	N/A
β-TrCP	5078981	laevis	+	+	+	–

cDNAs were screened first for their ability to be translated in rabbit reticulocyte lysate (RL), next their ability to bind SB-DNA beads (DNA binding), their preference for SB-DNA beads over DB-DNA beads (DSB-dep), and, finally, their sensitivity of DSB-binding to the prior depletion of Ku from extracts (Ku-dep). Proteins found to bind to SB-DNA beads are in bold.