Figure 6. Specific microRNA mimics modulated the expression of the Δ;Np63α protein interacting targets via their 3′-UTR sequences. (A) Predicted “seed” sequences for specific microRNAs in the protein target 3′-UTRs with the target prediction scores in parentheses. (B) SCC-11 cells were transfected with the LightSwitch_3UTR plasmids for the indicated protein 3′-UTRs along with the scrambled microRNA and mimics for miR-181a-5p, miR-374a-5p, miR-519-3p, miR-630 and miR-885-3p for 36 h. Cells were then tested for the RenSP Renilla luciferase reporter activity. Measurements (in triplicate) for the luciferase activity presented as relative units (RU). Values from cells transfected with the scrambled RNA were designated as 1.