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. 2013 Feb 1;10(2):192–204. doi: 10.4161/rna.23208

graphic file with name rna-10-192-g4.jpg

Figure 4. DSS1 enhances RNA editing in mammalian cells. (A) Comparison of editing levels in the RNAG editing reporter in HeLa cells. RNAG mRNA was isolated from HeLa cells transfected with an empty vector or a DSS1 expressing vector. RT-PCR with subsequent sequencing was performed. Shown is a graph with mean values of 3 runs, standard deviation and p-value as determined by student’s t-test. Also, a representative electropherogram is shown. (B) Stimulation of editing in a HeK293 cell line stably expressing rat(r)ADAR2. Anti-flag staining of Hek 293 cells with and without stable expression of rADAR2. (C) Comparison of editing levels at the K/E editing site in CyFip2 mRNA by direct sequencing of RT PCR products. Graphs with mean values of 3 independent runs, standard deviation and p-value as determined by standard deviation are shown. Also, a representative electropherogram is depicted.