Figure 5. Depletion of DSS1 and hnRNP A2/B1 leads to a reduction in RNA-editing. Short hairpin constructs directed against DSS1 or hnRNP A2/B1 were stably integrated in HeK293 cells expressing rat ADAR2 using lentiviral gene delivery. Stable lines showing a robust knockdown of DSS1 or hnRNP A2/B1 (see Fig. S2) were established. Editing levels of CyFIP2 and FLNa mRNAs were determined by direct sequencing of RT-PCR products. Knockdown of DSS1 leads to a reduction of CyFIP2 editing by 23% while depletion of hnRNPA2B1 reduces editing by 9%. Edited positions are marked by an arrowhead. Interestingly, editing of FLNa mRNA was also reduced by 23% upon DSS1 depletion but was not affected by hnRNP A2B1 depletion.