Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 1;10(2):228–244. doi: 10.4161/rna.23018

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2013 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

PMC Copyright notice

graphic file with name rna-10-228-g2.jpg — Figure 2. NOT2 and NOT3 interact via their C-terminal regions, which contain NOT-boxes. (A) NOT2 and NOT3 share a conserved C-terminal motif termed the NOT-box. NOT2 contains a less conserved N-terminal extension that is predicted to be unstructured (NOT2-N). NOT3 contains a highly conserved N-terminal domain (NOT3-N) that is connected to the NOT-box by a linker region (NOT3-L). (B) S2 cells coexpressing GFP-tagged NOT3 (full-length or fragments) and HA-tagged NOT2 were lysed 3 d after transfection. Cell lysates were immunoprecipitated using polyclonal anti-GFP antibodies and analyzed as described in Figure 1. (C and D) The interaction between GFP-tagged NOT2 (full-length or fragments) and HA-tagged NOT3 or NOT3-C was analyzed as described in Figure 1. (E) Interaction between recombinant NusA-tagged NOT3-C and GST-tagged NOT2-C. GST was used as a negative control.