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. 2013 Mar 11;8(3):e58500. doi: 10.1371/journal.pone.0058500

Figure 2. 3′ and 5′UTRs-driven regulation in control versus Ras-overexpressing cells.

Figure 2

a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “Materials and Methods”. The experiments were performed at least three times in quadruplicates (*p<0.05).