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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Comp Neurol. 2013 Jun 1;521(8):1803–1816. doi: 10.1002/cne.23260

Figure 8.

Figure 8

Larval photoreceptor basal bodies are randomly positioned. (A) Sagittal section through a 7 dpf Tg(-5actb2:cetn2-GFP)cu6 larvae shows the optic nerve (arrowhead) and larval retinal mosaic. (B) Enlarged view of sectioned retina shows how to determine the orientation of cells relative to the optic nerve (white circle). White arrows indicate the direction of the optic nerve, while red arrows indicate the 90° perpendicular angle. (C–D) Immunostaining with Zpr-1 (red) and GFP antibodies (yellow) identifies basal bodies in red-/green-sensitive cones. Blue-sensitive cones were negative for Zpr-1 and 4c12 immunoreactivity (arrows). (E) Inner and outer segments (autofluorescence from excitation with 488 nm light) and basal bodies (yellow) of UV-sensitive cones. (F) Basal bodies (yellow) and 4C12 (red) immunopositive rods. (G–J) The mean angular positions of individual fields of red-/green-, blue-, and UV-sensitive cones and rods are plotted on circular graphs. Grand mean angular positions are shown as black squares. In all images, nuclei are counterstained with DAPI (blue). Scale bar = 10 μm (C–F). Magenta-green copies are available in the Journal’s supplemental data figures